Rabbit Endothelial Nitric Oxide Synthase ELISA kit - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Rabbit Endothelial Nitric Oxide Synthase ELISA kit

E04N0024 Rabbit Endothelial Nitric Oxide Synthase ELISA kit

Rabbit Endothelial Nitric Oxide Synthase ELISA kit is suitable for detecting samples from rabbit species. Endothelial Nitric Oxide Synthase can also be called NOS-3, cNOS, Constitutive NOS, ecNOS, Endothelial nitric oxidase synthase, Endothelial nitric oxide synthase 3, Endothelial NOS, Nitric oxide synthase 3 (endothelial cell), Nitric oxide synthase 3, Nitric oxide synthase 3 endothelial cell, Nitric oxide synthase endothelial, nitric oxide synthase, endothelial, NOS 3, NOS III, NOS type III, eNOS.

Products

Specifications of Rabbit Endothelial Nitric Oxide Synthase ELISA kit

Product Information
Cat. No.	E04N0024
Product Name	Rabbit Endothelial Nitric Oxide Synthase ELISA kit
Species	Rabbit
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/mL
Sensitivity	1.0 pg/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

eNOS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- eNOS antibody and an eNOS-HRP conjugate. The assay sample and buffer are incubated together with eNOS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the eNOS concentration since eNOS from samples and eNOS-HRP conjugate compete for the anti-eNOS antibody binding site. Since the number of sites is limited, as more sites are occupied by eNOS from the sample, fewer sites are left to bind eNOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The eNOS concentration in each sample is interpolated from this standard curve.

Quality Control on Rabbit Endothelial Nitric Oxide Synthase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	89-114.79%
Linearity	Diluent Ratio	Range %
	1:2	96-116
	1:4	91-109
	1:8	88-103
Specificity/Cross-reactivity	No significant cross-reactivity or interference between eNOS and analogues was observed.