E04D0224 Rabbit D Dimer ELISA kit
Rabbit D Dimer ELISA kit is suitable for detecting samples from rabbit species. D Dimer can also be called D2D, D 2 Dimer, D-Dimer.
Rabbit D Dimer ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
D2D ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- D2D antibody and an D2D-HRP conjugate. The assay sample and buffer are incubated together with D2D-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the D2D concentration since D2D from samples and D2D-HRP conjugate compete for the anti-D2D antibody binding site. Since the number of sites is limited, as more sites are occupied by D2D from the sample, fewer sites are left to bind D2D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The D2D concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between D2D and analogues was observed.
E04D0224 has been referenced in the below publications:
The Study about Mechanism of Onion stalk extraction on Prevention and Treatment of Rabbits’Traumatic Deep Vein Thrombosis.
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