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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

E04R0005 Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit is suitable for detecting samples from rabbit species. Receptor Activator of Nuclear Factor Kb Ligand can also be called CD254, TNFSF11, ODF, OPGL, TRANCE, HRANKL2, SOdf, Tumor Necrosis Factor(ligand)superfamily Member 11, TNF-related activation-induced cytokine, RANKL.

Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Product Information

Cat. No.

E04R0005

Product Name

Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

0.5-10ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

RANKL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- RANKL antibody and an RANKL-HRP conjugate. The assay sample and buffer are incubated together with RANKL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RANKL concentration since RANKL from samples and RANKL-HRP conjugate compete for the anti-RANKL antibody binding site. Since the number of sites is limited, as more sites are occupied by RANKL from the sample, fewer sites are left to bind RANKL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RANKL concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between RANKL and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Summary of the Assay Procedure for Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Citations of Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

E04R0005 has been referenced in the below publications:

Effects of parathyroid hormone on OPG/RANKL/RANK signaling during distraction osteogenesis in a rabbit mandibular lengthening model.

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