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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Total Epidermal Growth Factor ELISA kit

E04E0032 Rabbit Total Epidermal Growth Factor ELISA kit

Rabbit Total Epidermal Growth Factor ELISA kit is suitable for detecting samples from rabbit species. Epidermal Growth Factor can also be called EGF; Beta urogastrone; Epidermal Growth Factor; Pro epidermal growth factor; URG; Urogastrone; HOMG4.

Specifications of Rabbit Total Epidermal Growth Factor ELISA kit

Product Information

Cat. No.

E04E0032

Product Name

Rabbit Total Epidermal Growth Factor ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

EGF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- EGF antibody and an EGF-HRP conjugate. The assay sample and buffer are incubated together with EGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EGF concentration since EGF from samples and EGF-HRP conjugate compete for the anti-EGF antibody binding site. Since the number of sites is limited, as more sites are occupied by EGF from the sample, fewer sites are left to bind EGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EGF concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Total Endothelin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between EGF and analogues was observed.


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Summary of the Assay Procedure for Rabbit Total Epidermal Growth Factor ELISA kit

Summary of the Assay Procedure for Rabbit Total Epidermal Growth Factor ELISA kit

Citations of Rabbit Total Epidermal Growth Factor ELISA kit

E04E0032 has been referenced in the below publications:

Effect of Banxia Houpu Decoction on EGF and GasConcentration in Peripheral Blood of Rabbits with Vomit Caused By Chemotherapy.

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