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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Brain Derived Neurotrophic Factor ELISA kit

E04B0029 Rabbit Brain Derived Neurotrophic Factor ELISA kit

Rabbit Brain Derived Neurotrophic Factor ELISA kit is suitable for detecting samples from rabbit species. Brain Derived Neurotrophic Factor can also be called BDNF, abrineurin, brain-derived neurotrophic factor, ANON2, BULN2, Brain-derived neurotrophic factor, brain derived neurotrophic factor, BDNF.

Specifications of Rabbit Brain Derived Neurotrophic Factor ELISA kit

Product Information

Cat. No.

E04B0029

Product Name

Rabbit Brain Derived Neurotrophic Factor ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

25-500 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

25 pg/mL

1 vial

STANDARD C (0.5mL)

50 pg/mL

1 vial

STANDARD D (0.5mL)

100 pg/mL

1 vial

STANDARD E (0.5mL)

250 pg/mL

1 vial

STANDARD F (0.5mL)

500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

BDNF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- BDNF antibody and an BDNF-HRP conjugate. The assay sample and buffer are incubated together with BDNF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BDNF concentration since BDNF from samples and BDNF-HRP conjugate compete for the anti-BDNF antibody binding site. Since the number of sites is limited, as more sites are occupied by BDNF from the sample, fewer sites are left to bind BDNF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BDNF concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Brain Derived Neurotrophic Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between BDNF and analogues was observed.


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Summary of the Assay Procedure for Rabbit Brain Derived Neurotrophic Factor ELISA kit

Summary of the Assay Procedure for Rabbit Brain Derived Neurotrophic Factor ELISA kit

Citations of Rabbit Brain Derived Neurotrophic Factor ELISA kit

E04B0029 has been referenced in the below publications:

Experiment study on the committed differentiation of adipose-derived stem cells in vitro and used into tissue-engineered peripheral nerves.

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