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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Cholesteryl Ester Transfer Protein ELISA kit

E04C0526 Rabbit Cholesteryl Ester Transfer Protein ELISA kit

Rabbit Cholesteryl Ester Transfer Protein ELISA kit is suitable for detecting samples from rabbit species. Cholesteryl Ester Transfer Protein can also be called BPIFF, HDLCQ10, cholesteryl ester transfer protein, plasma lipid transfer protein, CETP.

Specifications of Rabbit Cholesteryl Ester Transfer Protein ELISA kit

Product Information

Cat. No.

E04C0526

Product Name

Rabbit Cholesteryl Ester Transfer Protein ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

50-1000ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CETP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- CETP antibody and an CETP-HRP conjugate. The assay sample and buffer are incubated together with CETP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CETP concentration since CETP from samples and CETP-HRP conjugate compete for the anti-CETP antibody binding site. Since the number of sites is limited, as more sites are occupied by CETP from the sample, fewer sites are left to bind CETP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CETP concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Cholesteryl Ester Transfer Protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CETP and analogues was observed.


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Summary of the Assay Procedure for Rabbit Cholesteryl Ester Transfer Protein ELISA kit

Summary of the Assay Procedure for Rabbit Cholesteryl Ester Transfer Protein ELISA kit

Citations of Rabbit Cholesteryl Ester Transfer Protein ELISA kit

E04C0526 has been referenced in the below publications:

To explore the inhibition of Polyinosinic acid combined statin on Cholesterol crystals as well as expression of hs-crp in atherosclerotic rabbits.

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