E04P0034 Rabbit Platelet Derived Growth Factor AB ELISA kit
Rabbit Platelet Derived Growth Factor AB ELISA kit is suitable for detecting samples from rabbit species. Platelet Derived Growth Factor AB can also be called PDGF-AB, PDGF AB, PDGFAB.
Specifications of Rabbit Platelet Derived Growth Factor AB ELISA kit
Product Information | |
Cat. No. | E04P0034 |
Product Name | Rabbit Platelet Derived Growth Factor AB ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PDGF AB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- PDGF AB antibody and an PDGF AB-HRP conjugate. The assay sample and buffer are incubated together with PDGF AB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDGF AB concentration since PDGF AB from samples and PDGF AB-HRP conjugate compete for the anti-PDGF AB antibody binding site. Since the number of sites is limited, as more sites are occupied by PDGF AB from the sample, fewer sites are left to bind PDGF AB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDGF AB concentration in each sample is interpolated from this standard curve. |
Quality Control on Rabbit Platelet Derived Growth Factor AB ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PDGF AB and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rabbit Platelet Derived Growth Factor AB ELISA kit
Citations of Rabbit Platelet Derived Growth Factor AB ELISA kit
E04P0034 has been referenced in the below publications:
The effects of self-PRP on proliferation of BMSCs on titanium disk and the difference of PDGF.
