E04A1178 Rabbit Acetyl CoA acetyltransferase, mitochondrial ELISA kit
Rabbit Acetyl CoA acetyltransferase, mitochondrial ELISA kit is suitable for detecting samples from rabbit species. Acetyl CoA acetyltransferase, mitochondrial can also be called acetoacetyl-CoA thiolase, ACAT1, ACAT, MAT, T2, THIL, acetyl-CoA acetyltransferase 1, Acetyl-Coenzyme A acetyltransferase 1.
Product Information | |
Cat. No. | E04A1178 |
Product Name | Rabbit Acetyl CoA acetyltransferase, mitochondrial ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ACAT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- ACAT1 antibody and an ACAT1-HRP conjugate. The assay sample and buffer are incubated together with ACAT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACAT1 concentration since ACAT1 from samples and ACAT1-HRP conjugate compete for the anti-ACAT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACAT1 from the sample, fewer sites are left to bind ACAT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACAT1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ACAT1 and analogues was observed. | |
E04A1178 has been referenced in the below publications:
To explore the inhibition of Polyinosinic acid combined statin on Cholesterol crystals as well as expression of hs-crp in atherosclerotic rabbits.
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