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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Cross Linked C Telopeptide Of Type II Collagen ELISA kit

E04C0071 Rabbit Cross Linked C Telopeptide Of Type II Collagen ELISA kit

Rabbit Cross Linked C Telopeptide Of Type II Collagen ELISA kit is suitable for detecting samples from rabbit species. Cross Linked C Telopeptide Of Type II Collagen can also be called Cross Linked C-Telopeptide Of Type 2 Collagen ,CTX-2,Cross Linked C-Telopeptide Of Type II Collagen ,CTX-Ⅱ, CTx2, CTx 2.

Specifications of Rabbit Cross Linked C Telopeptide Of Type II Collagen ELISA kit

Product Information

Cat. No.

E04C0071

Product Name

Rabbit Cross Linked C Telopeptide Of Type II Collagen ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

250-5000pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CTx2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- CTx2 antibody and an CTx2-HRP conjugate. The assay sample and buffer are incubated together with CTx2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTx2 concentration since CTx2 from samples and CTx2-HRP conjugate compete for the anti-CTx2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CTx2 from the sample, fewer sites are left to bind CTx2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTx2 concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Cross Linked C Telopeptide Of Type II Collagen ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CTx2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Cross Linked C Telopeptide Of Type II Collagen ELISA kit

Summary of the Assay Procedure for Rabbit Cross Linked C Telopeptide Of Type II Collagen ELISA kit

Citations of Rabbit Cross Linked C Telopeptide Of Type II Collagen ELISA kit

E04C0071 has been referenced in the below publications:

The effects of immobilization and PTH on the capacity of spontaneous repair in an articular cartilage defect animal model.

The effets of microwave on osteoarthritis in knee of rabbits.

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