E04C0071 Rabbit Cross Linked C Telopeptide Of Type II Collagen ELISA kit
Rabbit Cross Linked C Telopeptide Of Type II Collagen ELISA kit is suitable for detecting samples from rabbit species. Cross Linked C Telopeptide Of Type II Collagen can also be called Cross Linked C-Telopeptide Of Type 2 Collagen ,CTX-2,Cross Linked C-Telopeptide Of Type II Collagen ,CTX-Ⅱ, CTx2, CTx 2.
Product Information | |
Cat. No. | E04C0071 |
Product Name | Rabbit Cross Linked C Telopeptide Of Type II Collagen ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CTx2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- CTx2 antibody and an CTx2-HRP conjugate. The assay sample and buffer are incubated together with CTx2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTx2 concentration since CTx2 from samples and CTx2-HRP conjugate compete for the anti-CTx2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CTx2 from the sample, fewer sites are left to bind CTx2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTx2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CTx2 and analogues was observed. | |
E04C0071 has been referenced in the below publications:
The effects of immobilization and PTH on the capacity of spontaneous repair in an articular cartilage defect animal model.
The effets of microwave on osteoarthritis in knee of rabbits.
Related Bluegene Biotech Products
![Rabbit NAD(P)H dehydrogenase [quinone] 1 ELISA kit](/uploads/image/20230423/14/bluegene-elisa-kit-25ng-ml-c_1682232377.webp "Rabbit NAD(P)H dehydrogenase [quinone] 1 ELISA kit")
