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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Lipocalin 2 ELISA kit

E04L0211 Rabbit Lipocalin 2 ELISA kit

Rabbit Lipocalin 2 ELISA kit is suitable for detecting samples from rabbit species. Lipocalin 2 can also be called Lipocalin-2, LCN2, p25, Lipocalin 2, Oncogene 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Siderocalin LCN2, LCN-2, LCN 2.

Specifications of Rabbit Lipocalin 2 ELISA kit

Product Information

Cat. No.

E04L0211

Product Name

Rabbit Lipocalin 2 ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

5.0-100ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

LCN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- LCN2 antibody and an LCN2-HRP conjugate. The assay sample and buffer are incubated together with LCN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LCN2 concentration since LCN2 from samples and LCN2-HRP conjugate compete for the anti-LCN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by LCN2 from the sample, fewer sites are left to bind LCN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LCN2 concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Lipocalin 2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between LCN2 and analogues was observed.


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Summary of the Assay Procedure for Rabbit Lipocalin 2 ELISA kit

Summary of the Assay Procedure for Rabbit Lipocalin 2 ELISA kit

Citations of Rabbit Lipocalin 2 ELISA kit

E04L0211 has been referenced in the below publications:

Comparison of two fluid solutions for resuscitation in a rabbit model of crush syndrome.

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