E04F0027 Rabbit Coagulation Factor XII ELISA kit
Rabbit Coagulation Factor XII ELISA kit is suitable for detecting samples from rabbit species. Coagulation Factor XII can also be called F12, HAE3, HAEX, HAF, coagulation factor XII, F12, F 12, F-12.
Rabbit Coagulation Factor XII ELISA kit
Product Information | |
Cat. No. | E04F0027 |
Product Name | Rabbit Coagulation Factor XII ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
F12 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- F12 antibody and an F12-HRP conjugate. The assay sample and buffer are incubated together with F12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the F12 concentration since F12 from samples and F12-HRP conjugate compete for the anti-F12 antibody binding site. Since the number of sites is limited, as more sites are occupied by F12 from the sample, fewer sites are left to bind F12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The F12 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rabbit Coagulation Factor XII ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between F12 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rabbit Coagulation Factor XII ELISA kit
Citations of Rabbit Coagulation Factor XII ELISA kit
E04F0027 has been referenced in the below publications:
Modulation of osteogenic and haemostatic activities by tuning cationicity of genipin-crosslinked chitosan hydrogels.
