Rabbit Collagen Type II Cleavage ELISA kit - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Rabbit Collagen Type II Cleavage ELISA kit

E04C0059 Rabbit Collagen Type II Cleavage ELISA kit

Rabbit Collagen Type II Cleavage ELISA kit is suitable for detecting samples from rabbit species. Collagen Type II Cleavage can also be called CIIC, C2C.

Products

Specifications of Rabbit Collagen Type II Cleavage ELISA kit

Product Information
Cat. No.	E04C0059
Product Name	Rabbit Collagen Type II Cleavage ELISA kit
Species	Rabbit
Product Size	48 Tests / 96 Tests
Concentration	10-250ng/mL
Sensitivity	1.0 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	10 ng/mL	1 vial
STANDARD C (0.5mL)	25 ng/mL	1 vial
STANDARD D (0.5mL)	50 ng/mL	1 vial
STANDARD E (0.5mL)	100 ng/mL	1 vial
STANDARD F (0.5mL)	250 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

C2C ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- C2C antibody and an C2C-HRP conjugate. The assay sample and buffer are incubated together with C2C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C2C concentration since C2C from samples and C2C-HRP conjugate compete for the anti-C2C antibody binding site. Since the number of sites is limited, as more sites are occupied by C2C from the sample, fewer sites are left to bind C2C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C2C concentration in each sample is interpolated from this standard curve.

Quality Control on Rabbit Collagen Type II Cleavage ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	89-114.79%
Linearity	Diluent Ratio	Range %
	1:2	96-116
	1:4	91-109
	1:8	88-103
Specificity/Cross-reactivity	No significant cross-reactivity or interference between C2C and analogues was observed.