Rabbit Oxidized lowdensity lipoprotein ELISA kit

BlueGene Biotech Rabbit Oxidized lowdensity lipoprotein ELISA kit

E04O0341 Rabbit Oxidized lowdensity lipoprotein ELISA kit

Rabbit Oxidized lowdensity lipoprotein ELISA kit is suitable for detecting samples from rabbit species. Oxidized lowdensity lipoprotein can also be called OL, OxLDL.

Products

Product Information
Cat. No.	E04O0341
Product Name	Rabbit Oxidized lowdensity lipoprotein ELISA kit
Species	Rabbit
Product Size	48 Tests / 96 Tests
Concentration	0.5-10ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

OxLDL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- OxLDL antibody and an OxLDL-HRP conjugate. The assay sample and buffer are incubated together with OxLDL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OxLDL concentration since OxLDL from samples and OxLDL-HRP conjugate compete for the anti-OxLDL antibody binding site. Since the number of sites is limited, as more sites are occupied by OxLDL from the sample, fewer sites are left to bind OxLDL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OxLDL concentration in each sample is interpolated from this standard curve.

Quality Control on Rabbit Oxidized lowdensity lipoprotein ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	89-114.79%
Linearity	Diluent Ratio	Range %
	1:2	96-116
	1:4	91-109
	1:8	88-103
Specificity/Cross-reactivity	No significant cross-reactivity or interference between OxLDL and analogues was observed.