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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Horse P selectin ELISA kit

E10P0111 Horse P selectin ELISA kit

Horse P selectin ELISA kit can be used to identify samples from the sheep species. P selectin can also be called CD62P, Selectin, Platelet, PSEL, GMP140, LECAM3, GRMP, GMRP, PADGEM, Leukocyte-endothelial cell adhesion molecule 3, Platelet activation dependent granule-external membrane, SELP.

Specifications of Horse P selectin ELISA kit

Product Information

Cat. No.

E10P0111

Product Name

Horse P selectin ELISA kit

Species

Horse

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SELP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SELP antibody and an SELP-HRP conjugate. The assay sample and buffer are incubated together with SELP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SELP concentration since SELP from samples and SELP-HRP conjugate compete for the anti-SELP antibody binding site. Since the number of sites is limited, as more sites are occupied by SELP from the sample, fewer sites are left to bind SELP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SELP concentration in each sample is interpolated from this standard curve.


Quality Control on Horse P selectin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SELP and analogues was observed.


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Summary of the Assay Procedure for Horse P selectin ELISA kit

Summary of the Assay Procedure for Horse P selectin ELISA kit

Citations of Horse P selectin ELISA kit

E10P0111 has been referenced in the below publications:

Prospective cohort study evaluating risk factors for the development of pasture-associated laminitis in the United Kingdom.

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