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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Horse Interferon γ ELISA kit

E10I0345 Horse Interferon γ ELISA kit

Horse Interferon γ ELISA kit can be used to identify samples from the Horse species. Interferon γ can also be called IFNγ, IFG, IFI, IFN Gamma, IFNG, IFN γ, IFN-γ, IFNGamma.

Specifications of Horse Interferon γ ELISA kit

Product Information

Cat. No.

E10I0345

Product Name

Horse Interferon γ ELISA kit

Species

Horse

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IFNγ ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IFNγ antibody and an IFNγ-HRP conjugate. The assay sample and buffer are incubated together with IFNγ-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNγ concentration since IFNγ from samples and IFNγ-HRP conjugate compete for the anti-IFNγ antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNγ from the sample, fewer sites are left to bind IFNγ-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNγ concentration in each sample is interpolated from this standard curve.


Quality Control on Horse Interferon γ ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IFNγ and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Horse Interferon γ ELISA kit

Summary of the Assay Procedure for Horse Interferon γ ELISA kit

Citations of Horse Interferon γ ELISA kit

E10I0345 has been referenced in the below publications:

Equine mesenchymal stromal cells and embryo-derived stem cells are immune privileged in vitro.

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