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E10A0530 Horse High Molecular Weight Adiponectin ELISA Kit

Horse High molecular weight Adiponectin ELISA kit is suitable for the detection of samples from Horse species. 

Specifications Of E10A0530 Horse HMW ADNP ELISA Kit

Porduct's Information

Cat.NO.

E10A0530

Porduct's Name

Horse High molecular weight Adiponectin ELISA kit

Species

Horse

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 μg/mL

Sensitivity

0.1 μg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 μg/mL

1 vial

STANDARD B (0.5mL)

0.5 μg/mL

1 vial

STANDARD C (0.5mL)

1.0 μg/mL

1 vial

STANDARD D (0.5mL)

2.5 μg/mL

1 vial

STANDARD E (0.5mL)

5.0 μg/mL

1 vial

STANDARD F (0.5mL)

10 μg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay
HMW ADNP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HMW ADNP antibody and an HMW ADNP-HRP conjugate. The assay sample and buffer are incubated together with HMW ADNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HMW ADNP concentration since HMW ADNP from samples and HMW ADNP-HRP conjugate compete for the anti-HMW ADNP antibody binding site. Since the number of sites is limited, as more sites are occupied by HMW ADNP from the sample, fewer sites are left to bind HMW ADNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMW ADNP concentration in each sample is interpolated from this standard curve.


Quality Control On Horse HMW ADNP ELISA Kit

CV

Intra Variation% <10%

Inter Variation% <12%

Recovery%

86-109.73

Linearity

Diluent ratio

Range %

1:2

91-109

1:4

89-112

1:8

87-115

Specificity/

Cross-reactivity

No significant cross-reactivity or interference between HMW  ADNP and analogues was observed.


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Summary Of The Assay Procedures For

Summary Of The Assay Procedures For

Citations Of Horse High Molecular Weight Adiponectin ELISA Kit

Validity and application of immunoturbidimetric and enzyme-linked immunosorbent assays for the measurement of adiponectin concentration in ponies


Glucagon-like peptide-1, insulin-like growth factor-1, and adiponectin in insulin-dysregulated ponies: effects of feeding a high nonstructural carbohydrate diet and association with prospective laminitis


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