E10A0530 Horse High Molecular Weight Adiponectin ELISA kit
Horse High molecular weight Adiponectin ELISA kit is suitable for the detection of samples from Horse species. High Molecular Weight Adiponectin can also be called HMW ADIPOQ, HMW ACDC, HMW ACRP30, HMW ADIPQTL1, HMW ADPN, HMW APM-1, HMW APM1, HMW GBP28, HMW adiponectin.
Product Information | |
Cat. No. | E10A0530 |
Product Name | Horse High molecular weight Adiponectin ELISA kit |
Species | Horse |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 μg/mL |
Sensitivity | 0.1 μg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 μg/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 μg/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 μg/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 μg/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 μg/mL | 1 vial |
STANDARD F (0.5mL) | 10 μg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
| Principle of the Assay |
| HMW ADNP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HMW ADNP antibody and an HMW ADNP-HRP conjugate. The assay sample and buffer are incubated together with HMW ADNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HMW ADNP concentration since HMW ADNP from samples and HMW ADNP-HRP conjugate compete for the anti-HMW ADNP antibody binding site. Since the number of sites is limited, as more sites are occupied by HMW ADNP from the sample, fewer sites are left to bind HMW ADNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMW ADNP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 86-109.73% | |
Linearity | Diluent Ratio | Range % |
1:2 | 91-109 | |
1:4 | 89-112 | |
1:8 | 87-115 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HMW ADNP and analogues was observed. | |
E10A0530 has been referenced in the below publications:
Validity and application of immunoturbidimetric and enzyme-linked immunosorbent assays for the measurement of adiponectin concentration in ponies.
Glucagon-like peptide-1, insulin-like growth factor-1, and adiponectin in insulin-dysregulated ponies: effects of feeding a high nonstructural carbohydrate diet and association with prospective laminitis.
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