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  • competitive elisa kit
  • competitive elisa kit

BlueGene Biotech Bovine Immunoglobulin G ELISA kit

NE11I0431 Bovine Immunoglobulin G ELISA kit

Bovine Immunoglobulin G ELISA kit is suitable for the detection of samples from bovine species. Immunoglobulin G can also be called as IgG.

Specifications of Bovine Immunoglobulin G ELISA kit

Product Information

Cat. NO.

NE11I0431

Product Name

Bovine Immunoglobulin G ELISA kit

Species

Bovine

Product Size

96 Tests

Concentration

78.125-5000 pg/ml

Sensitivity

26.8 pg/ml

Principal

Sandwich ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

3 hours and 15 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C



Kit Components

Reagents

Specification

Quantity

Pre-Coated Microplate (Detachable)

96 wells

1 plate (Keep Sealed)

Standard S1

5000pg/ml

1 vial

Standard S2

2500pg/ml

1 vial

Standard S3

1250pg/ml

1 vial

Standard S4

625pg/ml

1 vial

Standard S5

312.5pg/ml

1 vial

Standard S6

156.25pg/ml

1 vial

Standard S7

78.125pg/ml

1 vial

Standard S80pg/ml1 vial

Detection antibody (66×)

200ul

1 vial

Detection antibody Diluent

11ml

1 vial

HRP-Streptavidin (HRP, 200×)

100ul

1 vial

HRP-Streptavidin Diluent

11ml

1 vial

TMB Substrates

11ml

1 vial (Avoid Light)

Stop Solution

6ml

1 vial

Wash Solution (100×)

10ml

1 vial

Plate Sealer


4 pieces

Instruction Manual


1


Principle of the Assay

This ELISA kit is applied to the double antibody sandwich Enzyme Linked Immunosorbent Assay to detect the concentration of bovine IgG in samples. The microtiter plate has been pre-coated with rabbit anti-bovine antibody , standards or samples are then added to the microtiter plate wells and IgG if present, will bind to the antibody pre-coated wells under specific conditions. After washing, then a coupled biotin-conjugated detection rabbit anti-bovine is added to the wells, under 37℃ incubation, to form a precoated antibody-bovine IgG-detection antibody sandwich complex. After washing, coupled avidin conjugated Horseradish Peroxidase (HRP) is added to the wells, resulting in a cascade amplification effect. Then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total IgG bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of bovine IgG in samples is then determined by comparing the O.D. of the samples to the standard curve.


Quality Control on Bovine Immunoglobulin G ELISA kit

Coefficient of Variance

Intra Variation: 0-4%

Inter Variation: 5-13%

Recovery

65-100%

Linearity

Diluent Ratio

Range %

Average Linearity %

2.22×106

87.54-100.00

93.77

4.44×106

100.10-103.98

102.04

8.88×106

98.75-107.51

103.13

1.78×107

101.64-105.10

103.37

3.55×107

100.80-102.80

101.80

Specificity/

Cross-reactivity

Sample

Cross-Reactivity(%)

Mouse IgG

0.033

Goat IgG

0.266

Human IgG

0.019


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Immunoglobulin G ELISA kit VDR

Summary of the Assay Procedure for Bovine Immunoglobulin G ELISA kit VDR

Citations of Bovine Immunoglobulin G ELISA kit

Not available

Product: Not available

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