E02A0035 Rat Asymmetric Dimethylaoyoinine ELISA kit
Rat Asymmetric Dimethylaoyoinine ELISA kit is suitable for the detection of samples from Rat species. Asymmetric Dimethylaoyoinine can also be called N-Dimethylarginine, NG, NG-Dimethylarginine dihydrochloride, ADMA.
Product Information | |
Cat. No. | E02A0035 |
Product Name | Rat Asymmetric Dimethylaoyoinine ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ADMA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADMA antibody and an ADMA-HRP conjugate. The assay sample and buffer are incubated together with ADMA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADMA concentration since ADMA from samples and ADMA-HRP conjugate compete for the anti-ADMA antibody binding site. Since the number of sites is limited, as more sites are occupied by ADMA from the sample, fewer sites are left to bind ADMA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADMA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-109% | |
Linearity | Diluent Ratio | Range % |
1:2 | 91-103 | |
1:4 | 92-108 | |
1:8 | 87-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ADMA and analogues was observed. | |
E02A0031 has been referenced in the below publications:
Combined Intraperitoneal and Intrathecal Etanercept Reduce Increased Brain Tumor Necrosis Factor-Alpha and Asymmetric Dimethylarginine Levels and Rescues Spatial Deficits in Young Rats after Bile Duct Ligation.
Melatonin prevented spatial deficits and increases in brain asymmetric dimethylarginine in young bile duct ligation rats.
Study of the effect of ADMA on hepatic stellate cells proliferation and its possible signal pathway.
Impact of Rosuvastatin on vascular endothelial function in H-hypertensive rats.
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