E02C0235 Rat Pyridinoline cross linked carboxy terminal telopeptide of type I collagen ELISA kit ELISA Kit
The Rat Pyridinoline cross linked carboxy terminal telopeptide of type I collagen ELISA kit ELISA Kit can be used to identify samples from the rat species. Pyridinoline cross linked carboxy terminal telopeptide of type I collagen can also be called CTX, ICTP.
Product Information | |
Cat. No. | E02C0235 |
Product Name | Rat Pyridinoline cross linked carboxy terminal telopeptide of type I collagen ELISA kit ELISA Kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 25 pg/ml | 1 vial |
STANDARD C (0.5mL) | 50 pg/ml | 1 vial |
STANDARD D (0.5mL) | 100 pg/ml | 1 vial |
STANDARD E (0.5mL) | 250 pg/ml | 1 vial |
STANDARD F (0.5mL) | 500 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ICTP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ICTP. Standards or samples are then added to the microtiter plate wells and ICTP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ICTP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ICTP are added to each well to “sandwich” the ICTP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ICTP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ICTP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ICTP and analogues was observed. | |
E02C0235 has been referenced in the below publications：
Effects of the combined Herba Epimedii and Fructus Ligustri Lucidi on bone turnover and TGF-β1/Smads pathway in GIOP rats.
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