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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Anti thyroid globulin antibody ELISA kit (E02A0894)

E02A0894 Rat Anti thyroid globulin antibody ELISA kit

The Rat Anti thyroid globulin antibody ELISA kit can be used to identify samples from the rat species. Anti thyroid globulin antibody can also be called TGAb;AITD3Ab;TGNAb, thyroglobulin Ab, ATGA, TGAB, Anti-TG.

Products

Specifications of Rat Anti thyroid globulin antibody ELISA kit

Product Information

Cat. No.

E02A0894

Product Name

Rat Anti thyroid globulin antibody ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TGAB ELISA kit uses an anti-TGAB antibody and an TGAB-HRP conjugate in a competitive enzyme immunoassay method. TGAB-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TGAB antibody binding site between TGAB from samples and TGAB-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TGAB. Since the number of sites is limited, as more sites are occupied by TGAB from the sample, fewer sites are left to bind TGAB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGAB concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Anti thyroid globulin antibody ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TGAB and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Anti thyroid globulin antibody ELISA kit

Summary of the Assay Procedure for Rat Anti thyroid globulin antibody ELISA kit

Citations of Rat Anti thyroid globulin antibody ELISA kit

E02A0894 has been referenced in the below publications:

Oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female Wistar rats.

DINP aggravates autoimmune thyroid disease through activation of the Akt/mTOR pathway and suppression of autophagy in Wistar rats.


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