E03F0051 Mouse FAS Ligand ELISA kit
The Mouse FAS Ligand ELISA kit can be used to identify samples from the mouse species. FAS Ligand can also be called FASL, CD178, CD95L, CD95-L, FASLG, FASLG, APT1LG1, APT1-LG1, TNFSF6, Fas Antigen Ligand, TNF Superfamily Member 6, Tumor Necrosis Factor(ligand)Superfamily, Member 6．
Product Information | |
Cat. No. | E03F0051 |
Product Name | Mouse FAS Ligand ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
FASL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FASL antibody and an FASL-HRP conjugate. The assay sample and buffer are incubated together with FASL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FASL concentration since FASL from samples and FASL-HRP conjugate compete for the anti-FASL antibody binding site. Since the number of sites is limited, as more sites are occupied by FASL from the sample, fewer sites are left to bind FASL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FASL concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between FASL and analogues was observed. | |
E03F0051 has been referenced in the below publications：
Glucocorticoids impair oocyte developmental potential by triggering apoptosis of ovarian cells via activating the Fas system.
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