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  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Acetylcholine Receptor M1 ELISA kit (E03A0107)

E03A0107 Mouse Acetylcholine Receptor M1 ELISA kit

Mouse Acetylcholine Receptor M1 ELISA kit is suitable for the detection of samples from mouse species. Acetylcholine Receptor M1 can also be called CHR-M1, HM1, M1, M-AChRM1, Muscarinic Acetylcholine Receptor M1, Cholinergic Receptor, Muscarinic 1, CHRM1.

Products

Specifications of Mouse Acetylcholine Receptor M1 ELISA kit

Product Information

Cat. No.

E03A0107

Product Name

Mouse Acetylcholine Receptor M1 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

25-500 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AChR M1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AChR M1 antibody and an AChR M1-HRP conjugate. The assay sample and buffer are incubated together with AChR M1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AChR M1 concentration since AChR M1 from samples and AChR M1-HRP conjugate compete for the anti-AChR M1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AChR M1 from the sample, fewer sites are left to bind AChR M1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AChR M1 concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Acetylcholine Receptor M1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

94-113%

Linearity

Diluent Ratio

Range %

1:2

96-109

1:4

93-107

1:8

95-105

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AChR M1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Acetylcholine Receptor M1 ELISA kit

Summary of the Assay Procedure for Mouse Acetylcholine Receptor M1 ELISA kit

Citations of Mouse Acetylcholine Receptor M1 ELISA kit

E03A0107 has been referenced in the below publications:

Traditional Chinese medicine of peripheral neuropathy in diabetes rat sciatic nerve cell apoptosis and Bcl-2, Bax influence.

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