En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Interleukin 33 ELISA kit

E03I0204 Mouse Interleukin 33 ELISA kit

The Mouse Interleukin 33 ELISA kit can be used to identify samples from the mouse species. Interleukin 33 can also be called DV27, C9ORF26, IL1F11, NFHEV, Interleukin-1 Family, Member 11, Nuclear factor from high endothelial venules, IL 33.

Specifications of Mouse Interleukin 33 ELISA kit

Product Information

Cat. No.

E03I0204

Product Name

Mouse Interleukin 33 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/ml1 vial

STANDARD B (0.5mL)

50 pg/ml

1 vial

STANDARD C (0.5mL)

100 pg/ml

1 vial

STANDARD D (0.5mL)

250 pg/ml

1 vial

STANDARD E (0.5mL)

500 pg/ml

1 vial

STANDARD F (0.5mL)

1000 pg/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL 33 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 33 antibody and an IL 33-HRP conjugate. The assay sample and buffer are incubated together with IL 33-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 33 concentration since IL 33 from samples and IL 33-HRP conjugate compete for the anti-IL 33 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 33 from the sample, fewer sites are left to bind IL 33-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 33 concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Interleukin 33 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL 33 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Interleukin 33 ELISA kit

Summary of the Assay Procedure for Mouse Interleukin 33 ELISA kit

Citations of Mouse Interleukin 33 ELISA kit

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.