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BlueGene Biotech Mouse Amyloid β Protein 40 ELISA kit (E03A0049)

E03A0049 Mouse Amyloid β Protein 40 ELISA kit

Mouse Amyloid β Protein 40 ELISA kit is suitable for the detection of samples from mouse species. Amyloid β Protein 40 can also be called Aβ40, Amyloid beta 40, Abeta 40.

Products

Specifications of Mouse Amyloid β Protein 40 ELISA kit

Product Information

Cat. No.

E03A0049

Product Name

Mouse Amyloid β Protein 40 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Aβ40 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Aβ40 antibody and an Aβ40-HRP conjugate. The assay sample and buffer are incubated together with Aβ40-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Aβ40 concentration since Aβ40 from samples and Aβ40-HRP conjugate compete for the anti-Aβ40 antibody binding site. Since the number of sites is limited, as more sites are occupied by Aβ40 from the sample, fewer sites are left to bind Aβ40-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Aβ40 concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Amyloid β Protein 40 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-108%

Linearity

Diluent Ratio

Range %

1:2

95-109

1:4

91-101

1:8

92-106

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Aβ40 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Amyloid β Protein 40 ELISA kit

Summary of the Assay Procedure for Mouse Amyloid β Protein 40 ELISA kit

Citations of Mouse Amyloid β Protein 40 ELISA kit

E03A0049 has been referenced in the below publications:

Chronic noise exposure exacerbates AD-like neuropathology in SAMP8 mice in relation to Wnt signaling in the PFC and hippocampus.

Transcriptome analysis of the hippocampus in environmental noise-exposed SAMP8 mice reveals regulatory pathways associated with Alzheimer's disease neuropathology.

Environmental noise stress disturbs commensal microbiota homeostasis and induces oxi-inflammmation and AD-like neuropathology through epithelial barrier disruption in the EOAD mouse model.

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