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BlueGene Biotech Human Interleukin 8 ELISA kit (NE01I0056)

  • Immunology
  • Cardiovascular

NE01I0056 Human Interleukin 8 ELISA kit

Human Interleukin 8 ELISA kit is suitable for the detection of samples from human species. Interleukin 8 can also be called CXCL8, chemokine (C-X-C motif) ligand 8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, IL8, C-X-C motif chemokine ligand 8, Interleukin-8, SCYB8, IL-8, IL 8.

Products

Specifications of Human Interleukin 8 ELISA kit

Product Information

Cat. NO.

NE01I0056

Product Name

Human Interleukin 8 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

31.25-2000 pg/ml

Sensitivity

3.36pg/ml

Principal

Sandwich ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, and tissue homogenates

Assay Time

3 hours and 45 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

Reagents

Specification

Quantity

Pre-Coated Microplate (Detachable)

96 wells

1 plate (Keep Sealed)

Standard (Stock Solution, 20ng/ml)

150ul

1 vial

Standard Diluent S1

900ul

1 vial

Standard Diluent S2

500ul

1 vial

Standard Diluent S3

500ul

1 vial

Standard Diluent S4

500ul

1 vial

Standard Diluent S5

500ul

1 vial

Standard Diluent S6

500ul

1 vial

Standard Diluent S7

500ul

1 vial

Standard Diluent S8500ul1 vial

Detection antibody(50×)

160ul

1 vial

Detection antibody Diluent

6ml

1 vial

HRP-Streptavidin (150×)

100ul

1 vial

HRP-Streptavidin Diluent

10.5ml

1 vial

TMB Substrates

10.5ml

1 vial (Avoid Light)

Stop Solution

6ml

1 vial

Wash Solution (100×)

10ml

1 vial

Plate Sealer


4 pieces

Instruction Manual


1


Principle of the Assay

IL-8 ELISA kit is applied to the solid phase sandwich Enzyme Linked Immunosorbent Assay. The microtiter plate has been pre-coated with capture antibody which specific to IL-8. Standards or samples are then added to the microtiter plate wells and IL-8 if present, will bind to the antibody pre-coated wells. Then a biotin-conjugated detection antibody specific for IL-8 is added to the wells to form a capture antibody-antigen-detection antibody sandwich complex. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total IL-8 bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of IL-8 in samples is then determined by comparing the O.D. of the samples to the standard curve.


Quality Control on Human Interleukin 8 ELISA kit

Coefficient of Variance

Intra Variation: 0.8-5.5%

Inter Variation: 2.0-12%

Recovery

82-118 %(serum)

Linearity

Diluent Ratio

Range %

1:02

88-114

1:04

102-115

1:08

94-97

1:16

95-108

1:32

96-108

1:64

96-105

Specificity/

Cross-reactivity

Sample

Cross-Reactivity(%)

MMP-9

---

ErBb2

---

CECAM-7

---

Ang-1

---


Summary of the Assay Procedure for Human Interleukin 8 ELISA kit

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Citations of Human Interleukin 8 ELISA kit

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