Human Interleukin 8 ELISA kit is suitable for the detection of samples from human species. Interleukin 8 can also be called as CXCL8; chemokine (C-X-C motif) ligand 8; GCP-1; GCP1; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1; IL8, C-X-C motif chemokine ligand 8; Interleukin-8; SCYB8.
Specifications Of NE01I0056 Human IL-8 ELISA Kit
Porduct's Information | |
Cat.NO. | NE01I0056 |
Porduct's Name | Human Interleukin 8 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 31.25-2000 pg/ml |
Sensitivity | 3.36pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, and tissue homogenates |
Assay Time | 3 hours and 45 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 96 wells | 1 plate (Keep Sealed) |
Standard (Stock Solution, 20ng/ml) | 150ul | 1 vial |
Standard Diluent S1 | 900ul | 1 vial |
Standard Diluent S2 | 500ul | 1 vial |
Standard Diluent S3 | 500ul | 1 vial |
Standard Diluent S4 | 500ul | 1 vial |
Standard Diluent S5 | 500ul | 1 vial |
Standard Diluent S6 | 500ul | 1 vial |
Standard Diluent S7 Standard Diluent S8 | 500ul 500ul | 1 vial 1 vial |
Detection antibody(50×) | 160ul | 1 vial |
Detection antibody Diluent | 6ml | 1 vial |
HRP-Streptavidin (150×) | 100ul | 1 vial |
HRP-Streptavidin Diluent | 10.5ml | 1 vial |
TMB Substrates | 10.5ml | 1 vial (Avoid Light) |
Stop Solution | 6ml | 1 vial |
Wash Solution (100×) | 10ml | 1 vial |
Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
Principle of the Assay |
IL-8 ELISA kit is applied to the solid phase sandwich Enzyme Linked Immunosorbent Assay. The microtiter plate has been pre-coated with capture antibody which specific to IL-8. Standards or samples are then added to the microtiter plate wells and IL-8 if present, will bind to the antibody pre-coated wells. Then a biotin-conjugated detection antibody specific for IL-8 is added to the wells to form a capture antibody-antigen-detection antibody sandwich complex. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total IL-8 bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of IL-8 in samples is then determined by comparing the O.D. of the samples to the standard curve. |
Quality Control On Human IL-8 ELISA Kit
CV | Intra Variation%: 0.8-5.5 | |
Inter Variation%: 2.0-12 | ||
Recovery% | 82-118 (serum) | |
Linearity | Diluent ratio | Range % |
1:02 | 88-114 | |
1:04 | 102-115 | |
1:08 | 94-97 | |
1:16 | 95-108 | |
1:32 | 96-108 | |
1:64 | 96-105 | |
Specificity/ Cross-reactivity | Sample | Cross reactivity（%） |
MMP-9 | --- | |
ErBb2 | --- | |
CECAM-7 | --- | |
Ang-1 | --- | |
Summary Of The Assay Procedures For NE01I0056 Human IL-8 ELISA Kit
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