NE01L0038 Human Leptin ELISA kit
Human Leptin ELISA kit is suitable for the detection of samples from human species. Leptin can also be called LEP, LEPD, OB, OBS, leptin.
Specifications of NE01L0038 Human Leptin ELISA kit
Product Information | |
Cat. NO. | NE01L0038 |
Product Name | Human Leptin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 23.4-1500pg/ml |
Sensitivity | 13.2pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, and tissue homogenates |
Assay Time | 3 hours and 45 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 96 wells | 1 plate (Keep Sealed) |
Standard (Stock Solution, 15ng/ml) | 130ul | 1 vial |
Standard Diluent S1 | 900ul | 1 vial |
Standard Diluent S2 | 500ul | 1 vial |
Standard Diluent S3 | 500ul | 1 vial |
Standard Diluent S4 | 500ul | 1 vial |
Standard Diluent S5 | 500ul | 1 vial |
Standard Diluent S6 | 500ul | 1 vial |
Standard Diluent S7 | 500ul | 1 vial |
| Standard Diluent S8 | 500ul | 1 vial |
Detection antibody(150×) | 100ul | 1 vial |
Detection antibody Diluent | 10.5ml | 1 vial |
HRP-Streptavidin (150×) | 100ul | 1 vial |
HRP-Streptavidin Diluent | 10.5ml | 1 vial |
TMB Substrates | 10.5ml | 1 vial (Avoid Light) |
Stop Solution | 6ml | 1 vial |
Wash Solution (100×) | 10ml | 1 vial |
Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
Principle of the Assay |
Leptin ELISA kit applies the solid phase sandwich enzyme immunoassay technique. The microplate has been pre-coated with antibody specific to Leptin. Standards or samples are then added to the microplate wells and Leptin if present, will bind to the antibody pre-coated wells. Then a biotin-conjugated detection antibody specific to Leptin is added to the wells to form a pre-coatedantibody-antigen-detection antibody Complex. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns to blue after oxidating by HRP, and finally turns to yellow after adding the stop solution. Color develops in proportion to the amount of Leptin bound in theinitial steps. The color is measured spectrophotometrically at a wavelength of 450nm. The concentration of Leptin in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
Quality Control on Human Leptin ELISA kit
Coefficient of Variance | Intra Variation: 1.61-4.0% | |
Inter Variation: 2.4-5.8% | ||
Recovery | 111-120% (serum) | |
Linearity | Diluent Ratio | Range % |
1:02 | 104-106 | |
1:04 | 98-100 | |
1:08 | 99-100 | |
1:16 | 99-106 | |
1:32 | 99-111 | |
1:64 | 90-101 | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Leptin ELISA kit
Citations of Human Leptin ELISA kit
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