NE01I0004 Human/Canine/Porcine Insulin ELISA kit
Human/Canine/Porcine Insulin ELISA kit is suitable for the detection of samples from human species. Insulin can also be called ILPR, Insulin A chain, Insulin B chain, IRDN, INS, IDDM, IDDM1, IDDM2, ILPR, MODY10, insulin, PNDM4.
Product Information | |
Cat. NO. | NE01I0004 |
Product Name | Human/Canine/Porcine Insulin ELISA kit |
Species | Human/Canine/Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 7.81-500 pmol/L |
Sensitivity | 6.1pmol/L |
Principal | Sandwich ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, and tissue homogenates |
Assay Time | 2 hours and 45 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 96 wells | 1 plate (Keep Sealed) |
Standard (Stock Solution, 5000pmol/L) | 150ul | 1 vial |
Standard Diluent S1 | 900ul | 1 vial |
Standard Diluent S2 | 500ul | 1 vial |
Standard Diluent S3 | 500ul | 1 vial |
Standard Diluent S4 | 500ul | 1 vial |
Standard Diluent S5 | 500ul | 1 vial |
Standard Diluent S6 | 500ul | 1 vial |
Standard Diluent S7 | 500ul | 1 vial |
| Standard Diluent S8 | 500ul | 1 vial |
Detection antibody(60×) | 150ul | 1 vial |
Detection antibody Diluent | 6ml | 1 vial |
HRP-Streptavidin (150×) | 100ul | 1 vial |
HRP-Streptavidin Diluent | 10.5ml | 1 vial |
TMB Substrates | 11ml | 1 vial (Avoid Light) |
Stop Solution | 6ml | 1 vial |
Wash Solution (100×) | 10ml | 1 vial |
Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
Principle of the Assay |
This ELISA kit is applied to the solid phase sandwich Enzyme Linked Immunosorbent Assay. The microtiter plate has been pre-coated with capture antibody. The antigen to be measured and the biotin-conjugated detection antibody are then added to the microtiter plate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total insulin bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of insulin in samples is then determined by comparing the O.D. of the samples to the standard curve. |
Coefficient of Variance | Intra Variation: 3.01-6.35% | |
Inter Variation: 4.3-13% | ||
Recovery | 80-120%(serum) | |
Linearity | Diluent Ratio | Range % |
1:02 | 94-110 | |
1:04 | 98-105 | |
1:08 | 98-103 | |
1:16 | 97-106 | |
1:32 | 96-102 | |
1:64 | 98-103 | |
Specificity/ Cross-reactivity | Sample | Cross Reactivity（%） |
TfR | --- | |
IL-8 | --- | |
FGF | --- | |
IL-6 | --- | |
Not available
Product: Not available
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