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BlueGene Biotech Human Angiopoietin 2 ELISA kit

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  • Developmental Biology
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  • Metabolism

NE01A0503 Human Angiopoietin 2 ELISA kit

Human Angiopoietin 2 (ANG2) ELISA kit is suitable for the detection of samples from the human species. Angiopoietin 2 can also be called as AGPT2, ANGPT2,  ANG2, angiopoietin 2, and ANG-2.


Specifications of Human Angiopoietin 2 ELISA kit

Product's Information
Cat. NO.NE01A0503
Product NameHuman Heparin ELISA kit
Product Size48 Tests / 96 Tests
Concentration50-1000 ng/mL
PrincipalSandwich ELISA
Sample Volume100 ul
Sample Type

Serum, plasma, cell culture supernatants,

body fluid and tissue homogenate

Assay Time90 minutes
PlatformMicroplate Reader
Detection MethodColorimetric

Kit Components
Pre-Coated Microplate(Detachable)96 wells1 plate (Keep Sealed)
Standard (Stock Solution, 25ng/ml)220ul1 vial
Standard Diluent S1840ul1 vial
Standard Diluent S2500ul1 vial
Standard Diluent S3500ul1 vial
Standard Diluent S4500ul1 vial
Standard Diluent S5500ul1 vial
Standard Diluent S6500ul1 vial
Standard Diluent S7500ul1 vial
Standard Diluent S8500ul1 vial
Detection antibody(50×)270ul1 vial
Detection antibody Diluent11ml1 vial
HRP-Streptavidin (150×)100ul1 vial
HRP-Streptavidin Diluent10.5ml1 vial
TMB Substrates11ml1 vial (Avoid Light)
Stop Solution6ml1 vial
Wash Solution (100×)10ml1 vial
Sample's Diluent10ml2 vials
Instruction Manual

Principle of the Assay
ANG2 ELISA kit applies the solid phase sandwich enzyme immunoassay technique. The microplate has been pre-coated with antibody specific to ANG2. Standards or samples are then added to the microplate wells and ANG2 if present, will bind to the antibody pre-coated wells. Then a biotin-conjugateddetection antibody specific toANG2 is added to the wells to form a pre-coatedantibody-antigen-detection antibody Complex. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns to blue after oxidating by HRP, and finally turns to yellow after adding the stop solution. Color develops in proportion to the amount of ANG2 bound in theinitial steps. The color is measured spectrophotometrically at a wavelength of 450nm. The concentration of ANG2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Quality Control on Human Angiopoietin 2 ELISA kit

Coefficient of VarianceIntra Variation%: 5.7-9.5
Inter Variation%: 4.0-4.8
LinearityDiluent RatioRange %

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Summary of the Assay Procedure for Human Angiopoietin 2 ELISA kit

Summary of the Assay Procedure for Human Angiopoietin 2 ELISA kit

Citations of Human Angiopoietin 2 ELISA kit

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