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BlueGene Biotech Human C Reactive Protein ELISA kit (NE01C0009)

  • Immunology
  • Cardiovascular
  • Epigenetics and Nuclear Signaling
  • Metabolism
  • Neuroscience

NE01C0009 Human C Reactive Protein ELISA kit

Human C-reactive protein ELISA kit is suitable for the detection of samples from human species. C-reactive protein can also be called PTX 1, PTX1, C-reactive protein, pentraxin-related, C-reactive protein, CRP.

Products

Specifications of Human C Reactive Protein ELISA kit

Product Information

Cat. NO.

NE01C0009

Product Name

Human C-reactive protein ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

15.63-1000 pg/ml

Sensitivity

3.28pg/ml

Principal

Sandwich ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, and tissue homogenates

Assay Time

2 hours and 45 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

Reagents

Specification

Quantity

Pre-Coated Microplate (Detachable)

96 wells

1 plate (Keep Sealed)

Standard (Stock Solution, 12ng/ml)

170ul

1 vial

Standard Diluent S1

1100ul

1 vial

Standard Diluent S2

500ul

1 vial

Standard Diluent S3

500ul

1 vial

Standard Diluent S4

500ul

1 vial

Standard Diluent S5

500ul

1 vial

Standard Diluent S6

500ul

1 vial

Standard Diluent S7

500ul

1 vial

Standard Diluent S8500ul1 vial

Detection antibody(30×)

300ul

1 vial

Detection antibody Diluent

6ml

1 vial

HRP-Streptavidin (150×)

100ul

1 vial

HRP-Streptavidin Diluent

10.5ml

1 vial

TMB Substrates

11ml

1 vial (Avoid Light)

Stop Solution

6ml

1 vial

Wash Solution (100×)

10ml

1 vial

Plate Sealer


4 pieces

Instruction Manual


1


Principle of the Assay

CRP ELISA kit is appliedto the solid phase sandwich Enzyme Linked Immunosorbent Assay. The microtiter plate has been pre-coated with capture antibody which specific to CRP. Standards or samples are then added to the microtiter plate wells and CRP if present, will bind to the antibody pre-coated wells. Then a biotin-conjugated detection antibody specific for CRP is added to the wells to form a capture antibody-antigen-detection antibody sandwich complex. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total CRP bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of CRP in samples is then determined by comparing the O.D. of the samples to the standard curve.


Quality Control on Human C Reactive Protein ELISA kit

Coefficient of Variance

Intra Variation: 1.92-4.24%

Inter Variation: 4.51-13.11%

Recovery

82-97 (serum)%

Linearity

Diluent ratio

Range %

1:02

107.5-110.16

1:04

107.69-111.79

1:08

108.58-113.24

1:16

98.67-108.06

1:32

89.79-102.73

Specificity/

Cross-reactivity

Sample

Cross-Reactivity(%)

IL-6

---

CCL3

---

Mouse serum

---

Bovine serum

---


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human C Reactive Protein ELISA kit

Summary of the Assay Procedure for Human C Reactive Protein ELISA kit

Citations of Human C Reactive Protein ELISA kit

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