En
Email
  • competitive elisa kit
  • competitive elisa kit

BlueGene Biotech Human Interleukin 18 ELISA kit

  • Immunology
  • Cancer
  • Metabolism
  • Neuroscience

NE01I0310 Human Interleukin 18 ELISA kit

Human Interleukin 18 ELISA kit is suitable for the detection of samples from human species. Interleukin 18 can also be called interferon-gamma inducing factor, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma (IL-1 gamma), IL18, IL-18.

Specifications Of NE01I0310 Human IL-18 ELISA Kit

Porduct's Information

Cat.NO.

NE01I0310

Porduct's Name

Human Interleukin 18 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

15.63-1000 pg/ml

Sensitivity

5.66pg/ml

Principal

Sandwich ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, and tissue homogenates

Assay Time

2 hours and 45 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

Reagents

Specification

Quantity

Pre-Coated Microplate (Detachable)

96 wells

1 plate (Keep Sealed)

Standard (Stock Solution, 15ng/ml)

100ul

1 vial

Standard Diluent S1

980ul

1 vial

Standard Diluent S2

500ul

1 vial

Standard Diluent S3

500ul

1 vial

Standard Diluent S4

500ul

1 vial

Standard Diluent S5

500ul

1 vial

Standard Diluent S6

500ul

1 vial

Standard Diluent S7

Standard Diluent S8

500ul

500ul

1 vial

1 vial

Detection antibody(40×)

200ul

1 vial

Detection antibody Diluent

6ml

1 vial

HRP-Streptavidin (150×)

100ul

1 vial

HRP-Streptavidin Diluent

10.5ml

1 vial

TMB Substrates

11ml

1 vial (Avoid Light)

Stop Solution

6ml

1 vial

Wash Solution (100×)

10ml

1 vial

Plate Sealer


4 pieces

Instruction Manual


1


Principle of the Assay

IL-18 ELISA kit is applied to the solid phase sandwich Enzyme Linked Immunosorbent Assay. The microtiter plate has been pre-coated with capture antibody. The antigen to be measured and the biotin-conjugated detection antibody are then added to the microtiter plate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total IL-18 bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of IL-18 in samples is then determined by comparing the O.D. of the samples to the standard curve.


Quality Control On Human IL-18 ELISA Kit

CV

Intra Variation%: 5.2-12.2

Inter Variation%: 4.2-8.6

Recovery%

81-115 (serum)

Linearity

Diluent ratio

Range %

1:02

103.01-103.55

1:04

99.67-100.95

1:08

99.70-101.49

1:16

96.92-104.44

1:32

97.74-105.70

1:64

99.62-102.83

Specificity/

Cross-reactivity

Sample

Cross reactivity(%)

Pro-Collagen1α1

---

Ang-2

---

MMP-1

---

MMP-9

---


BlueGene Biotech Product Show

Summary Of The Assay Procedures For NE01I0310 Human IL-18 ELISA Kit

Summary Of The Assay Procedures For NE01I0310 Human IL-18 ELISA Kit

Publications Of Human Interleukin 18 ELISA Kit

Not available

Product: Not available

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.