NE01C0064 Human Chemokine (C-C motif) Ligand 18 ELISA Kit
Human Chemokine (C-C motif) ligand 18 ELISA kit is suitable for the detection of samples from human species. Chemokine (C-C motif) ligand 18 can also be called CCL18, chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated), AMAC-1, AMAC1, CKb7, DC-CK1, DCCK1, MIP-4, PARC, SCYA18, C-C motif chemokine ligand 18.
Product Information | |
Cat. NO. | NE01C0064 |
Products Name | Human Chemokine (C-C motif) ligand 18 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 31.25-2000 pg/ml |
Sensitivity | 4.02pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, and tissue homogenates |
Assay Time | 3 hours and 45 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 96 wells | 1 plate (Keep Sealed) |
Standard (Stock Solution, 20ng/ml) | 130ul | 1 vial |
Standard Diluent S1 | 900ul | 1 vial |
Standard Diluent S2 | 500ul | 1 vial |
Standard Diluent S3 | 500ul | 1 vial |
Standard Diluent S4 | 500ul | 1 vial |
Standard Diluent S5 | 500ul | 1 vial |
Standard Diluent S6 | 500ul | 1 vial |
Standard Diluent S7 | 500ul | 1 vial |
| Standard Diluent S8 | 500ul | 1 vial |
Detection antibody(100×) | 140ul | 1 vial |
Detection antibody Diluent | 10.5ml | 1 vial |
HRP-Streptavidin (150×) | 100ul | 1 vial |
HRP-Streptavidin Diluent | 10.5ml | 1 vial |
TMB Substrates | 10.5ml | 1 vial (Avoid Light) |
Stop Solution | 6ml | 1 vial |
Wash Solution (100×) | 10ml | 1 vial |
Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
Principle of the Assay |
CCL18 ELISA kit applies the solid phase sandwich enzyme immunoassay technique. The microplate has been pre-coated with antibody specific to CCL18. Standards or samples are then added to the microplate wells and CCL18 if present, will bind to the antibody pre-coated wells. Then a biotin-conjugated detection antibody specific to CCL18 is added to the wells to form a pre-coatedantibody-antigen-detection antibody Complex. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns to blue after oxidating by HRP, and finally turns to yellow after adding the stop solution. Color develops in proportion to the amount of CCL18 bound in theinitial steps. The color is measured spectrophotometrically at a wavelength of 450nm. The concentration of CCL18 in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
Coefficient of Variance | Intra Variation: 4.16-5.05% | |
Inter Variation: 4.1-8.2% | ||
Recovery | 95-107%(serum) | |
Linearity | Diluent Ratio | Range % |
1:02 | 103-120 | |
1:04 | 98-112 | |
1:08 | 101-110 | |
1:16 | 96-100 | |
1:32 | 103-122 | |
1:64 | 100-105 | |
Specificity/ Cross-reactivity | Sample | Cross-Reactivity（%） |
CCL3 | --- | |
Not available
Product: Not available
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