CHO Host Cell Protein OneStep ELISA Kit, G3 is for research use only; it should not be used in clinical diagnostic procedures. The kit is intended for the quantitative Measurement of CHO HCP Residues In Fermentation Broth, Purified Intermediates, Final Products and Cell Lysate.
Specifications of CH-K0018-3C1 CHO Host Cell Protein OneStep ELISA Kit, G3
| Product's Information | |
| Cat. NO. | CH-K0018-3C1 |
| Products Name | CHO Host Cell Protein OneStep ELISA Kit, G3 |
| Species | Chinese Hamster Ovary |
| Product Size | 96 Tests |
| Concentration | 0.78-200ng/ml |
| Sensitivity | LOD: 0.19 ng/ml ; LOQ: 0.78 ng/ml |
| Principal | Sandwich ELISA |
| Sample Volume | 100 ul |
| Sample Type | The kit is intended for the quantitative Measurement of CHO HCP Residues In Fermentation Broth, Purified Intermediates, Final Products and Cell Lysate. |
| Assay Time | 2 hours and 15 minutes |
| Platform | Microplate Reader |
| Conjugate | HRP |
| Detection Method | Colorimetric |
| Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 96 wells | 1 plate (Keep Sealed) |
Standard (Stock Solution) -500ug/ml | 50ul | 1 tube |
Detection antibody (100×) | 150ul | 1 tube |
TMB Substrates | 10ml | 1 vial (Avoid Light) |
Stop Solution | 10ml | 1 vial |
Wash Solution (20×) | 10ml | 5 vial |
Diluent Buffer (10×) | 10ml | 1 vial |
Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
This ELISA kit adopts to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with capture antibodies to prepare solid-phase antibodies. The sample antigen, and the HRP-conjugated detection antibodies are added simultaneously to the microplate wells, forming a coated antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by inputting the OD values of the samples into the standard curve equation, calculate the concentration of the target protein in the sample. |
Quality Control on CH-K0018-3C1 CHO Host Cell Protein OneStep ELISA Kit, G3
CV | Intra Variation%: 5-6 | |
Inter Variation%: 7-7.7 | ||
| Sensitivity | LOD: 0.19 ng/mL | |
LOQ: 0.78 ng/mL | ||
| Specificity | Samples | Concentration |
| HEK293 HCP | Not Detectable | |
| E.Coli HCP | Not Detectable | |
| Pichia Yeast HCP | Not Detectable | |
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Summary of The Assay Procedures for CH-K0018-3C1 CHO Host Cell Protein OneStep ELISA Kit, G3
