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  • ch k0018 1 cho host cell protein elisa kit
  • ch k0018 1 cho host cell protein elisa kit

BlueGene Biotech CHO Host Cell Protein ELISA Kit, G1

CHO Host Cell Protein ELISA Kit, G1 is for research use only, it should not be used in clinical diagnostic procedures. The kit is intended for the quantitative determination of host cell protein impurities in Cell culture supernatants or host cell protein (HCP) contamination in end-product expressed by Chinese Hamster Ovary (CHO) expression systems.

Specifications Of CH-K0018-1 CHO Host Cell Protein ELISA Kit, G1

Product's Information
Cat. NO.CH-K0018-1
Products NameCHO Host Cell Protein ELISA Kit, G1
Species
Product Size96 Tests
Concentration3.125-100ng/ml
SensitivityLOD: 0.2 ng/ml; LOQ: 3 ng/ml
PrincipalSandwich ELISA
Sample Volume100 ul
Sample Type

The kit is intended for the quantitative determination of host cell protein impurities in

Cell culture supernatants or host cell protein (HCP) contamination in end-product expressed

by Chinese Hamster Ovary (CHO) expression systems.

Assay Time3 hours and 10 minutes
PlatformMicroplate Reader
ConjugateHRP
Detection MethodColorimetric
Storage2-8°C



Kit Components

Reagents

Specification

Quantity

Pre-Coated Microplate (Detachable)

96 wells

1 plate (Keep Sealed)

Standard (Stock Solution) -500ug/ml

10ul

1 tube

Detection antibody (1400×)

15ul

1 tube

TMB Substrates

10ml

1 vial (Avoid Light)

Stop Solution

10ml

1 vial

Wash Solution (100×)

10ml

1 vial

Diluent Buffer (10×)

10ml

1 vial

Diluent Ingredient

0.5g

1 tube

Plate Sealer


4 pieces

Instruction Manual


1


Principle of the Assay

This ELISA kit is applied to the solid phase sandwich Enzyme Linked Immunosorbent Assay. The microtiter plate has been pre-coated with capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microtiter plate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with the antigen bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of ErbB2 in samples is then determined by comparing the O.D. of the samples to the standard curve.

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Quality Control On CH-K0018-1 CHO Host Cell Protein Detection Kit, G1

CV

Intra Variation%: 3.1-4.5

Inter Variation%: 6.0-9.1

Recovery%

95-110

Linearity

Diluent ratio

Range %

1:2

98.9-103.3

1:4

95.1-103

1:8

93.5-104.5

1:16

94.1-115.4

1:32

100-109.4


BlueGene Biotech Product Show

Summary Of The Assay Procedures For CH-K0018-1 CHO Host Cell Protein ELISA Kit, G1

Summary Of The Assay Procedures For CH-K0018-1 CHO Host Cell Protein ELISA Kit, G1

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