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BlueGene Biotech Human Thioredoxin reductase ELISA kit

E01T0922 Human Thioredoxin reductase ELISA kit

The Human Thioredoxin reductase ELISA kit can be used to identify samples from the human species. Thioredoxin reductase can also be called TR, TrxR, Thioredoxin reductase.

Specifications of Human Thioredoxin reductase ELISA kit

Product Information

Cat. No.

E01T0922

Product Name

Human Thioredoxin reductase ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TrxR ELISA kit uses an anti-TrxR antibody and an TrxR-HRP conjugate in a competitive enzyme immunoassay method. TrxR-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TrxR antibody binding site between TrxR from samples and TrxR-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TrxR. Since the number of sites is limited, as more sites are occupied by TrxR from the sample, fewer sites are left to bind TrxR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TrxR concentration in each sample is interpolated from this standard curve.


Quality Control on Human Thioredoxin reductase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TrxR and analogues was observed.


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Summary of the Assay Procedure for Human Thioredoxin reductase ELISA kit

Summary of the Assay Procedure for Human Thioredoxin reductase ELISA kit

Citations of Human Thioredoxin reductase ELISA kit

E01T0922 has been referenced in the below publications:

The role of oxidative stress caused by different feeding manners in the formation of emphysema in rats.


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