En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human High molecular weight Adiponectin ELISA kit (E01A0530)

E01A0530 Human High molecular weight Adiponectin ELISA kit

The Human High molecular weight Adiponectin ELISA kit can be used to identify samples from the human species. High molecular weight Adiponectin can also be called HMW ADIPOQ, HMW ACDC, HMW ACRP30, HMW ADIPQTL1, HMW ADPN, HMW APM-1, HMW APM1, HMW GBP28, HMW adiponectin.

Products

Specifications of Human High molecular weight Adiponectin ELISA kit

Product Information

Cat. No.

E01A0530

Product Name

Human High molecular weight Adiponectin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 µg/ml

Sensitivity

0.1 µg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 µg/mL

1 vial

STANDARD B (0.5mL)

1.0 µg/mL

1 vial

STANDARD C (0.5mL)

2.5 µg/mL

1 vial

STANDARD D (0.5mL)

5.0 µg/mL

1 vial

STANDARD E (0.5mL)

10 µg/mL

1 vial

STANDARD F (0.5mL)

25 µg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HMW ADNP ELISA kit uses an anti-HMW ADNP antibody and an HMW ADNP-HRP conjugate in a competitive enzyme immunoassay method. HMW ADNP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HMW ADNP antibody binding site between HMW ADNP from samples and HMW ADNP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HMW ADNP. Since the number of sites is limited, as more sites are occupied by HMW ADNP from the sample, fewer sites are left to bind HMW ADNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMW ADNP concentration in each sample is interpolated from this standard curve.


Quality Control on Human High molecular weight Adiponectin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HMW ADNP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human High molecular weight Adiponectin ELISA kit

Summary of the Assay Procedure for Human High molecular weight Adiponectin ELISA kit

Citations of Human High molecular weight Adiponectin ELISA kit

E01A0530 has been referenced in the below publications:

Changes of microalbuminuria and serum high molecular weight—adiponectin in hypertensive patients with metabolic syndrome.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.