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  • bovine serum albumin elisa 1

BlueGene Biotech Human Visceral adipose Tissue Derived Serine Protease Inhibitor ELISA kit (E01V0048)

E01V0048 Human Visceral adipose Tissue Derived Serine Protease Inhibitor ELISA kit

The Human Visceral adipose Tissue Derived Serine Protease Inhibitor ELISA kit can be used to identify samples from the human species. Visceral adipose Tissue Derived Serine Protease Inhibitor can also be called VASPIN, OL 64, OL-64, OL64, Serpin A12, Serpin A12 precursor, Serpina12, Ser A12, serpin family A member 12.

Products

Specifications of Human Visceral adipose Tissue Derived Serine Protease Inhibitor ELISA kit

Product Information

Cat. No.

E01V0048

Product Name

Human Visceral adipose Tissue Derived Serine Protease Inhibitor ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

VASPIN ELISA kit uses an anti-VASPIN antibody and an VASPIN-HRP conjugate in a competitive enzyme immunoassay method. VASPIN-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-VASPIN antibody binding site between VASPIN from samples and VASPIN-HRP conjugate, the intensity of the color is inversely proportional to the concentration of VASPIN. Since the number of sites is limited, as more sites are occupied by VASPIN from the sample, fewer sites are left to bind VASPIN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VASPIN concentration in each sample is interpolated from this standard curve.


Quality Control on Human Visceral adipose Tissue Derived Serine Protease Inhibitor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between VASPIN and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Visceral adipose Tissue Derived Serine Protease Inhibitor ELISA kit

Summary of the Assay Procedure for Human Visceral adipose Tissue Derived Serine Protease Inhibitor ELISA kit

Citations of Human Visceral adipose Tissue Derived Serine Protease Inhibitor ELISA kit

E01V0048 has been referenced in the below publications:

Plasma levels and placental expression of vaspin in pregnant women with diabetes mellitus.

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