E01I0310 Human Interleukin 18 ELISA kit
The Human Interleukin 18 ELISA kit can be used to identify samples from the human species. Interleukin 18 can also be called IL18, IL 18, IL-18, IL-1g, IGIF, interferon gamma-inducing factor IGIF, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, Iboctadekin, IFN gamma inducing factor, IL 1 gamma, IL 1g, IL1 gamma, IL18 protein, IL1F4, Interferon gamma inducing factor.
Product Information | |
Cat. No. | E01I0310 |
Product Name | Human Interleukin 18 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL18 ELISA kit uses an anti-IL18 antibody and a IL18-HRP conjugate in a competitive enzyme immunoassay method. IL18-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL18 antibody binding site between IL18 from samples and IL18-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL18. Since the number of sites is limited, as more sites are occupied by IL18 from the sample, fewer sites are left to bind IL18-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL18 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL18 and analogues was observed. | |
E01I0310 has been referenced in the below publications：
Role of NLRP3 inflammasome in endothelial cells inflammation induced by high uric acid.
The clinical significance of detection the Interleukin-18 and NO change in the serum of chronic severe hepatitis B patients and the relevant studies with entecavir treatment.
The study of the correlation between dendritic cell and the degree of carotid atherosclerosis and the stability of atherosclerosis plaques.
Influence of NLRP3，AIM2 and CASP1 genes in acute exacerbations of chronic obstructive pulmonary diseases.
The urinary concentration change of acute kidney biomarker in coronary artery angiography and percutaneous coronary intervention therapy patient.
Analysis of atrial fibrillation associated with high-sensitivity C-reactive protein and interleukin-18 in Qinghai region.
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