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  • bovine serum albumin elisa 1

BlueGene Biotech Human Bone Morphogenetic Protein 9 ELISA kit

E01B0391 Human Bone Morphogenetic Protein 9 ELISA kit

The Human Bone Morphogenetic Protein 9 ELISA kit can be used to identify samples from the human species. Bone Morphogenetic Protein 9 can also be called BMP9, GDF2, BMP-9, BMP9, HHT5, GDF-2, growth differentiation factor 2.

Specifications of Human Bone Morphogenetic Protein 9 ELISA kit

Product Information

Cat. No.

E01B0391

Product Name

Human Bone Morphogenetic Protein 9 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

BMP9 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BMP9. Standards or samples are then added to the microtiter plate wells and BMP9 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BMP9 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for BMP9 are added to each well to “sandwich” the BMP9 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound coBMP9nents. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BMP9 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP9 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Bone Morphogenetic Protein 9 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between BMP9 and analogues was observed.


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Summary of the Assay Procedure for Human Bone Morphogenetic Protein 9 ELISA kit

Summary of the Assay Procedure for Human Bone Morphogenetic Protein 9 ELISA kit

Citations of Human Bone Morphogenetic Protein 9 ELISA kit

E01B0391 has been referenced in the below publications:

RESEARCH ABNORMAL BLOOD SUGAR IN HEAVY MEDITTERRANEAN ANEMIA PATIENTS AFTER HEMATOPOIETIC STEM CELL TRANSPLANTION AND RELATED INFLUENCE FACTORS.

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