E01L0207 Human Lipoarabinomannan ELISA kit
The Human Lipoarabinomannan ELISA kit can be used to identify samples from the human species. Lipoarabinomannan can also be called LAM.
Human Lipoarabinomannan ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
LAM ELISA kit uses an anti-LAM antibody and an LAM-HRP conjugate in a competitive enzyme immunoassay method. LAM-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LAM antibody binding site between LAM from samples and LAM-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LAM. Since the number of sites is limited, as more sites are occupied by LAM from the sample, fewer sites are left to bind LAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LAM concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between LAM and analogues was observed.
E01L0207 has been referenced in the below publications：
Values of Early Secretory Antigenic Target 6 and Lipoarabinomannan in Diagnosis of Tuberculous Meningitis.
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