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BlueGene Biotech Human Tumor Necrosis Factor β ELISA kit (E01T0021)

E01T0021 Human Tumor Necrosis Factor β ELISA kit

The Human Transglutaminase ELISA kit can be used to identify samples from the human species. Transglutaminase can also be called TNFβ, LTA, LT, TNFB, TNFSF1, Lymphotoxin alpha, TNLG1E, TNF-B, Tumor Necrosis Factor Ligand Superfamily Member 1,Lymphotoxin Alpha.

Products

Specifications of Human Tumor Necrosis Factor β ELISA kit

Product Information

Cat. No.

E01T0021

Product Name

Human Tumor Necrosis Factor β ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TNFβ ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TNFβ. Standards or samples are then added to the microtiter plate wells and TNFβ if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TNFβ present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TNFβ are added to each well to “sandwich” the TNFβ immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TNFβ and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNFβ concentration in each sample is interpolated from this standard curve.


Quality Control on Human Tumor Necrosis Factor β ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TNFβ and analogues was observed.


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Summary of the Assay Procedure for Human Tumor Necrosis Factor β ELISA kit

Summary of the Assay Procedure for Human Tumor Necrosis Factor β ELISA kit

Citations of Human Tumor Necrosis Factor β ELISA kit

E01T0021 has been referenced in the below publications:

淋巴瘤患者血浆TNF-α和TNF-β水平及其临床意义。

Myeloid cell-like transcript 2 is related to liver inflammation and the pathogenesis of hepatitis B via the involvement of CD8+T cell activation.

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