E01M0209 Human Macrophage MigRation Inhibitory Factor ELISA kit
The Human Macrophage MigRation Inhibitory Factor ELISA kit can be used to identify samples from the human species. Macrophage MigRation Inhibitory Factor can also be called Macrophage Migration Inhibitory Factor, GIF, GLIF, Glycosylation inhibiting factor, MIF protein, MMIF, Phenylpyruvate tautomerase, acrophage migration inhibitory factor (glycosylation-inhibiting factor), L-dopachrome isomerase.
Product Information | |
Cat. No. | E01M0209 |
Product Name | Human Macrophage MigRation Inhibitory Factor ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MIF ELISA kit uses an anti-MIF antibody and an MIF-HRP conjugate in a competitive enzyme immunoassay method. MIF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MIF antibody binding site between MIF from samples and MIF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MIF. Since the number of sites is limited, as more sites are occupied by MIF from the sample, fewer sites are left to bind MIF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIF concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MIF and analogues was observed. | |
E01M0209 has been referenced in the below publications：
Effects of atorvastatin given before acute PCI on MCP-1,MIF and prognosis of patients with STEMI.
Dengue Virus Nonstructural Protein 1 Induces Vascular Leakage through Macrophage Migration Inhibitory Factor and Autophagy.
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