En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Aspartate Aminotransferase ELISA kit

E01A0626 Human Aspartate Aminotransferase ELISA kit

The Human Aspartate Aminotransferase ELISA kit can be used to identify samples from the human species. Aspartate Aminotransferase can also be called AST, Aspartate transaminase, aspartate aminotransferase, AspAT/ASAT/AAT, (serum) glutamic oxaloacetic transaminase (GOT, SGOT).

Specifications of Human Aspartate Aminotransferase ELISA kit

Product Information

Cat. No.

E01A0626

Product Name

Human Aspartate Aminotransferase ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AST ELISA kit uses an anti-AST antibody and an AST-HRP conjugate in a competitive enzyme immunoassay method. AST-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-AST antibody binding site between AST from samples and AST-HRP conjugate, the intensity of the color is inversely proportional to the concentration of AST. Since the number of sites is limited, as more sites are occupied by AST from the sample, fewer sites are left to bind AST-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AST concentration in each sample is interpolated from this standard curve.


Quality Control on Human Aspartate Aminotransferase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AST and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Aspartate Aminotransferase ELISA kit

Summary of the Assay Procedure for Human Aspartate Aminotransferase ELISA kit

Citations of Human Aspartate Aminotransferase ELISA kit

E01A0626 has been referenced in the below publications:

Clinical application analysis of ECG and cardiac enzymes histological changes in acute cerebrovascular disease.

贵州贵阳107例甲型病毒性肝炎暴发流行性调查。

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.