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BlueGene Biotech Human Interleukin 4 ELISA kit

E01I0007 Human Interleukin 4 ELISA kit

The Human Interleukin 4 ELISA kit can be used to identify samples from the human species. Interleukin 4 can also be called Interleukin-4, IL4, IL 4, IL-4, Interleukin 4.

Specifications of Human Interleukin 4 ELISA kit

Product Information

Cat. No.

E01I0007

Product Name

Human Interleukin 4 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL4. Standards or samples are then added to the microtiter plate wells and IL4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL4 are added to each well to “sandwich” the IL4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL4 concentration in each sample is interpolated from this standard curve. 


Quality Control on Human Interleukin 4 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL4 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Interleukin 4 ELISA kit

Summary of the Assay Procedure for Human Interleukin 4 ELISA kit

Citations of Human Interleukin 4 ELISA kit

E01I0007 has been referenced in the below publications:

Immune-modulation of cord blood-derived multipotent stem cells on 1ymphocytes of patients with Alzheimer’s disease.

Effects of IL-8 on the function of human umbilical vein endothelial cell line(HUVEC).

变应性鼻炎患者鼻黏膜中脑源性神经营养因子(BDNF)和IL-4表达 的意义及相关性研究。

The Clinical research of Ma Xing Gan Shi treating Pathogenic heat which Basing on the mode of Sequential administration.

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