E01I0007 Human Interleukin 4 ELISA kit
The Human Interleukin 4 ELISA kit can be used to identify samples from the human species. Interleukin 4 can also be called Interleukin-4, IL4, IL 4, IL-4, Interleukin 4.
Specifications of Human Interleukin 4 ELISA kit
Product Information | |
Cat. No. | E01I0007 |
Product Name | Human Interleukin 4 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL4. Standards or samples are then added to the microtiter plate wells and IL4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL4 are added to each well to “sandwich” the IL4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL4 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Interleukin 4 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL4 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Interleukin 4 ELISA kit
Citations of Human Interleukin 4 ELISA kit
E01I0007 has been referenced in the below publications：
Immune-modulation of cord blood-derived multipotent stem cells on 1ymphocytes of patients with Alzheimer’s disease.
Effects of IL-8 on the function of human umbilical vein endothelial cell line(HUVEC).
变应性鼻炎患者鼻黏膜中脑源性神经营养因子(BDNF)和IL-4表达 的意义及相关性研究。
The Clinical research of Ma Xing Gan Shi treating Pathogenic heat which Basing on the mode of Sequential administration.
