E01L0038 Human Leptin ELISA kit
The Human Leptin ELISA kit can be used to identify samples from the human species. Leptin can also be called LEP, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, OB, Obese Protein, Obesity, Obesity factor, Obesity homolog mouse, Obesity Murine Homolog Leptin, OBS.
Product Information | |
Cat. No. | E01L0038 |
Product Name | Human Leptin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Leptin ELISA kit uses an anti-Leptin antibody and an Leptin-HRP conjugate in a competitive enzyme immunoassay method. Leptin-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Leptin antibody binding site between Leptin from samples and Leptin-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Leptin. Since the number of sites is limited, as more sites are occupied by Leptin from the sample, fewer sites are left to bind Leptin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Leptin concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Leptin and analogues was observed. | |
E01L0038 has been referenced in the below publications：
Correlation of serum osteopontin and leptin levels with femoral head necrosis.
Study of Pioglitazone Reducing Coronary In-stent Restenosis on Insulin Resistance in Non-diabetic Patients.
The change of Leptin in GCF before and after initial therapy of Chronic periodontitis.
Acupuncture Improves Intestinal Absorption of Iron in Iron‑deficient Obese Patients: A Randomized Controlled Preliminary Trial.
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