E01L0022 Human Low density lipoprotein cholesterol ELISA kit
The Human Low density lipoprotein cholesterol ELISA kit can be used to identify samples from the human species. Low density lipoprotein cholesterol can also be called LDL C.
Specifications of Human Low density lipoprotein cholesterol ELISA kit
Product Information | |
Cat. No. | E01L0022 |
Product Name | Human Low density lipoprotein cholesterol ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LDL C ELISA kit uses an anti-LDL C antibody and an LDL C-HRP conjugate in a competitive enzyme immunoassay method. LDL C-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LDL C antibody binding site between LDL C from samples and LDL C-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LDL C. Since the number of sites is limited, as more sites are occupied by LDL C from the sample, fewer sites are left to bind LDL C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LDL C concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Low density lipoprotein cholesterol ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LDL C and analogues was observed. | |
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Summary of the Assay Procedure for Human Low density lipoprotein cholesterol ELISA kit
Citations of Human Low density lipoprotein cholesterol ELISA kit
E01L0022 has been referenced in the below publications：
Relationship between Klotho gene polymorphism and serum levels of Klotho protein in patients with early renal damage in essential hypertension patients.
