En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Total Cholesterol ELISA kit (E01T0020)

E01T0020 Human Total Cholesterol ELISA kit

The Human Total Cholesterol ELISA kit can be used to identify samples from the human species. Cholesterol can also be called TC, Cholesterin, Cholesteryl alcohol.

Products

Specifications of Human Total Cholesterol ELISA kit

Product Information

Cat. No.

E01T0020

Product Name

Human Total Cholesterol ELISA kit 

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 mmol/L 

Sensitivity

0.1 mmol/L 

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 mmol/L 

1 vial

STANDARD B (0.5mL)

2.5 mmol/L 

1 vial

STANDARD C (0.5mL)

5.0 mmol/L 

1 vial

STANDARD D (0.5mL)

10 mmol/L 

1 vial

STANDARD E (0.5mL)

25 mmol/L 

1 vial

STANDARD F (0.5mL)

50 mmol/L 

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TC ELISA kit uses an anti-TC antibody and an TC-HRP conjugate in a competitive enzyme immunoassay method. TC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TC antibody binding site between TC from samples and TC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TC. Since the number of sites is limited, as more sites are occupied by TC from the sample, fewer sites are left to bind TC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TC concentration in each sample is interpolated from this standard curve.


Quality Control on Human Total Cholesterol ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TC and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Total Cholesterol ELISA kit

Summary of the Assay Procedure for Human Total Cholesterol ELISA kit

Citations of Human Total Cholesterol ELISA kit

E01T0020 has been referenced in the below publications:

Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.