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BlueGene Biotech Human Surfactant Protein J ELISA kit (E01S0096)

E01S0096 Human Surfactant Protein J ELISA kit

The Human Surfactant Protein J ELISA kit can be used to identify samples from the human species. Surfactant Protein J can also be called SPJ, SPG, Surfactant Associated Protein G, Surfactant-associated protein 2, SFTA2.

Products

Specifications of Human Surfactant Protein J ELISA kit

Product Information

Cat. No.

E01S0096

Product Name

Human Surfactant Protein J ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1 ng/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SPJ ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SPJ. Standards or samples are then added to the microtiter plate wells and SPJ if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SPJ present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SPJ are added to each well to “sandwich” the SPJ immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SPJ and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPJ concentration in each sample is interpolated from this standard curve.


Quality Control on Human Surfactant Protein J ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SPJ and analogues was observed.


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Summary of the Assay Procedure for Human Surfactant Protein J ELISA kit

Summary of the Assay Procedure for Human Surfactant Protein J ELISA kit

Citations of Human Surfactant Protein J ELISA kit

E01S0096 has been referenced in the below publications:

Surfactant proteins A and D are related to severity of the disease, pathogenic bacteria and comorbidity in patients with chronic rhinosinusitis with and without nasal polyps.

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