E01H0238 Human High density lipoprotein cholesterol ELISA kit
The Human High density lipoprotein cholesterol ELISA kit can be used to identify samples from the human species. High density lipoprotein cholesterol can also be called HDL C, HDL-C.
Specifications of Human High density lipoprotein cholesterol ELISA kit
Product Information | |
Cat. No. | E01H0238 |
Product Name | Human High density lipoprotein cholesterol ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 µmol/L |
Sensitivity | 0.1 µmol/L |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 µmol/L | 1 vial |
STANDARD B (0.5mL) | 1.0 µmol/L | 1 vial |
STANDARD C (0.5mL) | 2.5 µmol/L | 1 vial |
STANDARD D (0.5mL) | 5.0 µmol/L | 1 vial |
STANDARD E (0.5mL) | 10 µmol/L | 1 vial |
STANDARD F (0.5mL) | 25 µmol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HDL C ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HDL C. Standards or samples are then added to the microtiter plate wells and HDL C if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HDL C present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HDL C are added to each well to “sandwich” the HDL C immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HDL C and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HDL C concentration in each sample is interpolated from this standard curve. |
Quality Control on Human High density lipoprotein cholesterol ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HDL C and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human High density lipoprotein cholesterol ELISA kit
Citations of Human High density lipoprotein cholesterol ELISA kit
E01H0238 has been referenced in the below publications：
Relationship between Klotho gene polymorphism and serum levels of Klotho protein in patients with early renal damage in essential hypertension patients.
