Human Advanced Glycosylation End Products ELISA Kit is suitable for the detection of samples from human species. Advanced Glycosylation End Products can also be known as Advanced Glycation End Product; AGE.
Specifications of E01A0002 Human AGEs ELISA Kit
Porduct's Information | |
Cat.NO. | E01A0002 |
Porduct's Name | Human Advanced Glycosylation End Products ELISA Kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AGES ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AGES. Standards or samples are then added to the microtiter plate wells and AGES if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AGES present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AGES are added to each well to “sandwich” the AGES immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AGES and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGES concentration in each sample is interpolated from this standard curve. |
Quality Control On Human AGEs ELISA Kit
CV | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery% | 93-106 | |
Linearity | Diluent ratio | Range % |
1:2 | 94-104 | |
1:4 | 90-109 | |
1:8 | 92-105 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AGEs and analogues was observed. | |
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Summary of The Assay Procedures For E01A0002 Human AGEs ELISA Kit
Citations of Human Advanced Glycosylation End Products ELISA Kit
The correlation between aspirin resistance in patients with Diabetes and AGEs and HOMA-IR
Eplerenone restores 24-h blood pressure circadian rhythm and reduces advanced glycation endproducts in rhesus macaques with spontaneous hypertensive metabolic syndrome
Product: E01S0283 Human Solube receptor for advanced glycation end products ELISA kit
Ellagic acid reveals promising anti-aging effects against d-galactose-induced aging on human neuroblastoma cell line, SH-SY5Y: A mechanistic study
