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BlueGene Biotech Human High-temperature requirement factor A4 ELISA kit (E01H1404)

E01H1404 Human High-temperature requirement factor A4 ELISA kit

The Human High-temperature requirement factor A4 ELISA kit can be used to identify samples from the human species. High-temperature requirement factor A4 can also be called HTRA4.

Products

Specifications of Human High-temperature requirement factor A4 ELISA kit

Product Information

Cat. No.

E01H1404

Product Name

Human High-temperature requirement factor A4 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HTRA4 ELISA kit uses an anti-HTRA4 antibody and an HTRA4-HRP conjugate in a competitive enzyme immunoassay method. HTRA4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HTRA4 antibody binding site between HTRA4 from samples and HTRA4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HTRA4. Since the number of sites is limited, as more sites are occupied by HTRA4 from the sample, fewer sites are left to bind HTRA4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HTRA4 concentration in each sample is interpolated from this standard curve.


Quality Control on Human High-temperature requirement factor A4 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HTRA4 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human High-temperature requirement factor A4 ELISA kit

Summary of the Assay Procedure for Human High-temperature requirement factor A4 ELISA kit

Citations of Human High-temperature requirement factor A4 ELISA kit

E01H1404 has been referenced in the below publications:

Study of Htr A4 and Syncytin-1 in Preeclampsia.

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